Expert of Antibody Production and Genetic Modification

Anti-CB1 (Mouse) Monoclonal Antibody

Concentration: 1.0 mg/mL
Physical State: Liquid
Size: 50μg

The cannabinoid receptor type 1 is a guanine-nucleotide-binding protein (G-protein)-coupled cannabinoid receptor primarily located in the central and peripheral nervous system. It is activated by endo-cannabinoid neurotransmitters; by plant cannabinoids including THC, an active ingredient of the psychoactive drug cannabis and by synthetic analogues of THC. Two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

Gene Name

Cannabinoid Receptor 1

Gene ID

NCBI Reference Sequence: NM_007726.3

Family

GPCR, Cannabinoid

Synonyms

CB1; CNR1 Antibody; CB-R Antibody; CB1A Antibody; CB1R Antibody; CANN6 Antibody; CB1K5 Antibody

Host

Mouse

Clonality

Monoclonal

Clone ID

IMG-3C2

Isotype

IgG1

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 443-473.

Mouse 

MHRAAESCIKSTVKIAKVTMSVSTDTSAEAL

Human

VHRAAESCIKSTVKIAKVTMSVSTDTSAEAL

Species Reactivity

Mouse, Human (and with species that show high homology, although not tested)

Buffer

0.02M Potassium Phosphate, 0.15M Sodium Chloride pH 7.2

Stabilizer

10mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free

Preservative

0.01% (w/v) Sodium Azide

Storage Condition

For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This monoclonal antibody is suitable for Immunohistochemistry (IHC) (paraffin embedded (IHC-P) or fresh frozen (IHC-F) tissue specimen and western blot. Expect a ~52.8kDa single band corresponding to the CB1 protein by western blot when analyzing appropriate cell lysates or extracts. This CB1 monoclonal antibody specifically reacts with mouse and human CB1, while cross-reactivity with CB1 protein originating from different species and/or other sources has not been determined. Specific conditions for reactivity should be optimized by the end user.

Purity / Specificity

This product was purified from concentrated tissue culture supernatant by Protein G chromatography and specifically reacts with human CB1 protein.

Assay Dilution

User Optimized

  • Immunocytochemistry (ICC) 100 ng/ml
  • Immunofluorescence (IF) 1 μg/ml
  • OTHER ASSAYS User Optimized

Figure 1: FcRn TG mouse anti-CB1 monoclonal antibody IMG-3C2 reacts to a highly conserved C-terminal 31-mer oligopeptide of the mouse cannabinoid receptor type 1 (CB1R) that is identical to its human ortholog as shown by immunofluorescence using the antibody at 1 μg/ml. (Figure is reproduced with permission from Dr István Katona, Institute of Experimental Medicine, Hungarian Academy of Sciences).

 

Figure 1

 

Laboratory Reagent For In Vitro Research Use Only
Not for resale without prior written consent from ImmunoGenes AG.