Expert of Antibody Production and Genetic Modification

Anti-CB1 (Rabbit) Polyclonal Antibody

Concentration: 1.0 μg/mL
Physical State: Liquid
Size: 50μg

The cannabinoid receptor type 1 is a guanine-nucleotide-binding protein (G-protein)-coupled cannabinoid receptor primarily located in the central and peripheral nervous system. It is activated by endo-cannabinoid neurotransmitters; by plant cannabinoids including THC, an active ingredient of the psychoactive drug cannabis and by synthetic analogues of THC. Two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.



http://www.immunogenes.com/featuredproducts/polyclonal/picture1.jpg
 

Figure 1: Immunostaining with the affinity purified anti-CB1 (rabbit) polyclonal antibody developed by ImmunoGenes against CB1 receptor labels axon terminals of hippocampal interneurons. (a) Confocal image showing the biocytin-labeled axon and axon terminals of an interneuron recorded in acute brain slice. (b) Subsequent STORM super-resolution imaging revealed CB1R immunolabeling on the identified axon terminals. (c) Magnified view of the STORM localization points from the boxed region in b depicts the nanoscale distribution of CB1 receptors. (Reference: Dudok et al. 2014. Cell-Specific STORM Super-Resolution Imaging Reveals Nanoscale Organization Of Cannabinoid Signaling. Nat Neurosci 2014 Dec 8. Doi: 10.1038/Nn.3892).

 

Gene Name

Cannabinoid Receptor 1

Gene ID

NCBI Reference Sequence: NM_007726.3

Family

GPCR, Cannabinoid

Synonyms

CB1; CNR1 Antibody; CB-R Antibody; CB1A Antibody; CB1R Antibody; CANN6 Antibody; CB1K5 Antibody

Host

Rabbit

Clonality

Polyclonal

Immunogen

This polyclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 443-473.

Mouse 

MHRAAESCIKSTVKIAKVTMSVSTDTSAEAL

Human

VHRAAESCIKSTVKIAKVTMSVSTDTSAEAL

Species Reactivity

Mouse, Human (and with species that show high homology, although not tested)

Buffer

0.02M Potassium Phosphate, 0.15M Sodium Chloride pH 7.2

Stabilizer

10mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free

Preservative

0.01% (w/v) Sodium Azide

Storage Condition

For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This polyclonal antibody is suitable for Immunohistochemistry (IHC) (paraffin embedded (IHC-P) or fresh frozen (IHC-F) tissue specimen and western blot. Expect a ~52.8kDa single band corresponding to the CB1 protein by western blot when analyzing appropriate cell lysates or extracts. This CB1 polyclonal antibody specifically reacts with mouse and human CB1, while cross-reactivity with CB1 protein originating from different species and/or other sources has notbeen determined. Specific conditions for reactivity should be optimized by the end user.

Purity / Specificity

This product was purified from rabbit serum by peptide immunoaffinity chromatography and specifically reacts with human CB1 protein.

Assay Dilution

User Optimized

  • Immunocytochemistry (ICC) 1 μg/ml
  • Immunofluorescence (IF) 1 μg/ml
  • Immunohistochemistry (IHC) 1 μg/ml
  • OTHER ASSAYS User Optimized

Reference

Dudok et al. 2014. Cell-Specific STORM Super-Resolution Imaging Reveals Nanoscale Organization Of Cannabinoid Signaling. Nat Neurosci 2014 Dec 8. Doi: 10.1038/Nn.3892

Laboratory Reagent For In Vitro Research Use Only
Not for resale without prior written consent from ImmunoGenes AG.