Expert of Antibody Production and Genetic Modification

Anti-Insulin (Mouse) Monoclonal Antibody

Anti-Insulin_(Mouse)_Monoclonal_Antibody.jpg Human insulin (INS) monoclonal antibody may be used for the analysis of the structure, function and metabolism of insulin. This anti-insulin antibody, is applicable for immune-histological studies of tissue sections.

Figure 1 .Staining of 4% paraformaldehyde-fixed pancreas tissue sections (50 µm thick) with 1:100 anti-insulin monoclonal antibody (IMG-7B2) for 3 nights at 4°C using Cy3-conjugated donkey anti-mouse secondary antibody (Jackson Immunoresearch, 1:500).

Antigenic specificity

Insulin (INS)

Host

Mouse

Clonality

IMG-7B2, Monoclonal (IgG2a kappa)

Concentration, package size

1 mg/ml (liquid), 100 μg

Immunogen

This monoclonal antibody was produced by repeated immunizations with a KLH-conjugated synthetic peptide corresponding to the C-terminal part of human insulin chain B

Species Reactivity

Human, Rat (and other species that show high homology, although not tested)

Buffer

0.02M Potassium Phosphate, 0.15M Sodium Chloride pH 7.2

Stabilizer

10 mg/ml Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free

Preservative

0.01% (w/v) Sodium Azide

Storage Condition

For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

This monoclonal antibody is suitable for Immunohistochemistry (IHC) (paraffin embedded (IHC-P) or fresh frozen (IHC-F) tissue specimen and ELISA. Specific conditions for reactivity should be optimized by the end user.

Purity / Specificity

This product was purified from concentrated tissue culture supernatant by Protein G chromatography and specifically reacts with human insulin protein.

Assay Dilution

Immunocytochemistry (ICC) 1:100 Immunohistochemistry (IHC) 1:100 Other assays user optimized