Expert of Antibody Production and Genetic Modification

Higher antigen specific immunoglobulin titers

FcRn Overexpression – higher antigen specific immunoglobulin titers and more antigen-specific B-cells

FcRn overexpression results in increased antigen-specific IgM, IgG, and total IgG levels and number of antigen-specific B-cells

 

A

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B

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C

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D

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Tg and wild-type mice were immunized with ovalbumin (OVA) (A) OVA-specific IgM and (B) OVAspecific IgG titers were nearly tripled in Tg mice compared with the wild-type (wt) animals. (C) Tg mice produced significantly higher amounts of total IgG compared to the wt mice. (D) Larger spleen and multiple-fold increase of OVA-specific IgM and IgG producer cells was detected in Tg mice compared with wt controls (Cervenak et al. 2011. The Journal of Immunology).