Expert of Antibody Production and Genetic Modification

Breaking immune tolerance

FcRn Overexpression – allow production of antibodies against of highly conserved antigens - breaking immune tolerance

Immunization with highly conserved mouse cannabinoid receptor type 1 (CB1; GPCR) resulted in CB1-specific mAbs only in bFcRn Tg mice (C-terminal mouse CB1 specific oligopeptide conjugated to KLH; this segment is identical to its human ortholog)

 

Immuntolerance.jpg

Mouse Type CB1 Positives Clones
(IgG)
Balb/c 0
bFcRn Tg 10