About Us

Benefits of IMG-AbS™

FcRn Overexpression – more efficient antigen presentation, increased antibody diversity

bFcRn is strongly expressed in Tg DCs and MΦs

 

Figure 1

Western Blot

Figure 1

qpcr

 

Western blot analysis shows the expression of bovine α-chain protein in spleen (TgS), B cells (B) T cells (T), peritoneal neutrophil granulocytes (Ne), bone marrow derived dendritic cells (DC), and peritoneal macrophages (MΦ) from non-immunized bFcRn Tg animals. Based on its molecular weight, the 38-kDa band was specific for bFcRn as also evidenced by its expression in the bFcRn stable transfected cells (B4) for positive and spleen (WtS) from a control BALB/c mouse as negative control, respectively. Blot was stripped and rehybridized with an anti-β-actin monoclonal antibody as a loading control. qPCR analysis show expression ratios of the bFcRn to mFcRn at mRNA level in MΦ, DCs and spleen from bFcRn Tg mice analyzed with a quantitative real-time PCR assay. We found that the bFcRn expression was 3.8-, 2.6- and 10-fold higher compared to the expression of the mFcRn in spleen, MΦ, DCs, respectively (Vegh et al. 2012. PLoS One).

bFcRn Tg macrophages and dendritic cells phagocytose antigen-IgG immune complex more efficiently and antigen-IgG immune complex loaded dendritic cells stimulate T cells more efficiently as compared to cells from wt mice

A


A continued

 

A cont

 

B


B

C


B

(A) Peritoneal macrophages (MΦ) and (B) bone marrow-derived dendritic cells (DCs) isolated from bFcRn Tg and wt mice were incubated with Alexa-conjugated ovalbumin(OVA)-IgG immune complex (IC) or with Alexa-conjugated OVA alone for the indicated times. The uptake was analyzed by flow cytometry. (A. cont) Peritoneal MΦ from bFcRn Tg and wt mice were incubated with Alexa-conjugated OVA-IgG (green) at 37°C for 60 min. Cells were then washed, and labeled with anti-CD11b (red) and visualized by confocal microscopy. (C) Bone marrow-derived DCs from wt and bFcRn Tg mice were left untreated or loaded with either OVA-IgG IC or OVA alone. CD4+ T cells from OVA TCR (DO11.10) Tg mice were then added to both the untreated and loaded DCs. After 24 hours, proliferating T cells were labeled for 12 h with [H3]-thymidine. As a positive control, CD4+ T cells were incubated with Concavalin A (ConA) (Vegh et al. 2012. PLoS One).